Advantages of TransAM Transcription Factor ELISAs

Historically, transcription factor studies have been conducted using EMSAs/gel shift assays, western blots, and reporter-based assays with plasmid transfections, which are all time-consuming, do not allow for high-throughput analysis and provide only semi-quantitative results.

TransAM assays are up to 100 times more sensitive than gel shifts and can be completed in fewer than 5 hours. The TransAM assay is a non-radioactive ELISA-based assay, making them easier to perform. The high-throughput stripwell format enables simultaneous screening of up to 96 samples. Inconsistencies due to variable reporter plasmid transfection efficiencies are also eliminated, along with the need to construct stable cell lines.

Why Use TransAM Over Traditional Methods?

• Sensitivity: Up to 100-fold more sensitive than gel shift assays

• Convenience: Eliminates the use of radioactivity and the need to run gels

• It's Fast: Results can be obtained in fewer than 5 hours

• Ease of Analysis: Colorimetric readout enables simple quantitative analysis with spectrophotometry at 450 nm

• Flexible Format: 96-stripwell format enables both high- and low-throughput assays

Our TransAM Assay kits have been cited in more than 1,000 publications.