Prestained Protein Ladder – Broad molecular weight (10-245 kDa)

Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE(Tris-glycine buffer).

This prestained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The protein ladder is supplied in gel loading buffer and is ready to use.

  • Form

    Liquid

  • Storage instructions

    Please see notes section.

  • Storage buffer

    pH: 7.5
    Constituents: 0.44% Tris citrate/phosphate, 0.02% Urea, 2% Sodium lauryl sulfate, 0.02% DTT, 15% Glycerol

  • Broad range: 10-245 kDa

  • Ready-to-use: Supplied in a loading buffer for direct loading on gels.

  • Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.

  • Sharp bands

  • Recommended loading: ~1.5 - 2.5µl

  • Application: WB, SDS-PAGE.