FACE™ In-cell Western Phospho ELISAs

FACE™ In-cell Western Phospho ELISAs

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Fast Activated Cell-based ELISA (FACE™ In-Cell Western) Kits provide a simple, sensitive method for detecting protein phosphorylation directly in the cell, without making extracts or performing electrophoresis and membrane blotting. These 96-well, high-throughput In-Cell ELISA assays are available in both colorimetric and chemiluminescent formats for over 20 different targets (see list at right). If you need an In-Cell ELISA Kit for a target that is not currently offered, you can use our FACE Maker In-Cell Western ELISA Kits to create your own FACE assays.

Why use FACE™ In-Cell Western ELISA Kits?

  • Simple, non-radioactive method that is easily quantified

  • Fixing preserves activation-specific protein modifications

  • Cell-based assay eliminates the need to make cell extracts

  • Minimal hands-on time – less than 3 hours

  • Two primary antibodies included to compare phosphorylated and native protein

  • Increased linear analysis range

  • Compatible with high-throughput automation.