Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Format

96T

Detection range

15.6-1,000pg/mL

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 5.7pg/mL.

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay length

3h

Method

Double-antibody Sandwich ELISA (sELISA).

Specificity

This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Beta (IL1b).
No significant cross-reactivity or interference between Interleukin 1 Beta (IL1b) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Shipping & Storage

Shipped at 4°C. 

For unused kit: The whole kit could be stored at -20^oC in shelf life, while up to one month at 4^oC. For experiment convenience, reagents could also be stored seperately, Standard, Detection Reagent A, Detection Reagent B and 96-well strip plate should be stored at -20^oC while the others could be at 4 ^oC.

Citations of Primary Manufacturer