ELISA Kit for Human Creatine Kinase MB Isoenzyme (CKMB)

The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CKMB in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Item Name

Creatine Kinase MB Isoenzyme (CKMB)

Alternative Names

CK2; CK-MB; Creatine Kinase,Muscle/Brain

Organism Species
Homo sapiens (Human) 
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
Detection Range
The minimum detectable dose of this kit is typically less than 0.60ng/mL.

This assay has high sensitivity and excellent specificity for detection of Creatine Kinase MB Isoenzyme (CKMB).
No significant cross-reactivity or interference between Creatine Kinase MB Isoenzyme (CKMB) and analogues was observed.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Creatine Kinase MB Isoenzyme (CKMB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Creatine Kinase MB Isoenzyme (CKMB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Shipping & Storage

Shipped at 4°C. 

For unused kit: The whole kit could be stored at -20oC in shelf life, while up to one month at 4oC. For experiment convenience, reagents could also be stored seperately, Standard, Detection Reagent A, Detection Reagent B and 96-well strip plate should be stored at -20oC while the others could be at 4 oC.

Citations of Primary Manufacturer

Magazine Citations
Cardiovascular Toxicology Aldehyde Dehydrogenase-2 Activation during Cardioplegic Arrest Enhances the Cardioprotection against Myocardial Ischemia–Reperfusion Injury PubMed: 22814936
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Int J Clin Exp Med Effects of morphine and sufentanil preconditioning against myocardial ischemic-reperfusion injury in rabbits PubMed: 26629064
European Journal of Pharmacology Diabetes blocks the cardioprotective effects of sevoflurane postconditioning by impairing Nrf2/Brg1/HO-1 signaling Pubmed:26973173
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American Journal of Translational Research Peroxisome proliferator-activated receptor γ (PPARγ) mediates the protective effect of quercetin against myocardial ischemia-reperfusion injury via … PMC5209473
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Archivos de medicina veterinaria Cardiac biomarkers in dogs with visceral leishmaniasis S0301-732X2016000300004
Cellular Physiology and Biochemistry Remote Limb Ischaemic Postconditioning Protects Against Myocardial Ischaemia/ Reperfusion Injury in Mice: Activation of JAK/STAT3-Mediated Nrf2-Antioxidant Signalling 10.1159:481755
Erciyes Medical Journal Morinda Citrifolia (Noni) and Low Dose Aspirin Prevent Apoptotic Cell Death and Oxidative Stress on Isoproterenol Induced Myocardial Infarction in Rats DOI: 10.5152/etd.2017.1732
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Science of the Total Environment Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity and Pubmed:29727991
Journal of Surgical Research GYY4137 protects against myocardial ischemia/reperfusion injury via activation of the PHLPP-1/Akt/Nrf2 signaling pathway in diabetic mice Pubmed:29605032
Molecular Medicine Reports  Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats Pubmed:29956781
Cell Death Discovery Cyclic helix B peptide ameliorates acute myocardial infarction in mice by inhibiting apoptosis and inflammatory responses
Oxidative Medicine and Cellular Longevity Adiponectin Facilitates Postconditioning Cardioprotection through Both AMPK-Dependent Nuclear and AMPK-Independent Mitochondrial STAT3 Activation Pubmed: 32190173
PLoS One Tissue suction-mediated gene transfer to the beating heart in mice Pubmed: 32027678
FASEB JOURNAL Deletion of Rap1 protects against myocardial ischemia/reperfusion injury through suppressing cell apoptosis via activation of STAT3 signaling Pubmed: 32020680
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In Vivo Hydrogen sulphide and nitric oxide cooperate in cardioprotection against ischemia/reperfusion injury in isolated rat heart Pubmed: 32871779
Drug Design Development and Therapy Melatonin Attenuates Anoxia/Reoxygenation Injury by Inhibiting Excessive Mitophagy Through the MT2/SIRT3/FoxO3a Signaling Pathway in H9c2 Cells Pubmed: 32546969
Nanomaterials Biological Safety and Biodistribution of Chitosan Nanoparticles Pubmed: 32340313
Molecular Medicine Reports The mRNA expression of Il6 and Pdcd1 are predictive and protective factors for doxorubicin?induced cardiotoxicity 33300057
Oxid Med Cell Longev Inactivation of TOPK Caused by Hyperglycemia Blocks Diabetic Heart Sensitivity to Sevoflurane Postconditioning by Impairing the PTEN/PI3K/Akt Signaling 33986917
Eur J Pharmacol Long non-coding RNA MALAT1 modulates myocardial ischemia-reperfusion injury though the PI3K/Akt/eNOS pathway by sponging miRNA-133a-3p to target IGF1R … Pubmed:34968461
Cardiovasc Drugs Ther Hyperglycemia-Induced Overexpression of PH Domain Leucine-Rich Repeat Protein Phosphatase 1 (PHLPP1) Compromises the Cardioprotective Effect of Ischemic … Pubmed:35715527
Nutrients Mokko Lactone Alleviates Doxorubicin-Induced Cardiotoxicity in Rats via Antioxidant, Anti-Inflammatory, and Antiapoptotic Activities. Nutrients 2022, 14, 733 Pubmed:35215383
Experimental and Therapeutic Medicine Hydrogen therapy after resuscitation improves myocardial injury involving inhibition of autophagy in an asphyxial rat model of cardiac arrest Pubmed:35495584
Oxidative Medicine and Cellular Longevity MicroRNA-503 Exacerbates Myocardial Ischemia/Reperfusion Injury via Inhibiting PI3K/Akt-and STAT3-Dependent Prosurvival Signaling Pathways Pubmed:35620576
International Journal of Sciences: Basic and Applied Research Vitamin E Attenuates Cardiomyopathy Via Alleviation of Autophagic Stress