Cell-Free DNA (cfDNA) Purification Kit

Cell-Free DNA (cfDNA) Purification Kit

Code: 25503 & 25504

Cell-free DNA (cfDNA) circulates in blood plasma outside of cells, and has recently been used as a powerful sample type for disease diagnostics. Epigenetic marks found on cfDNA, such as DNA methylation, can be used as biomarkers for cancer, pregnancy, and aging, to name a few. However, this powerful method is inherently limited by the amount of cfDNA that can be isolated from a blood draw. Therefore, it is important to maximize retention of cfDNA when purifying from a sample.

The Cell-Free DNA (cfDNA) Purification Kit is designed to efficiently isolate cell-free DNA from human plasma and serum samples. Using Active Motif's fast, magnetic bead-based protocol, it is easy to isolate and concentrate cell-free DNA from fresh or frozen samples. The profile below demonstrates total elimination of high molecular weight genomic DNA from the sample, leaving behind the signature bimodal size distribution profile of cfDNA.

Contents & Storage

The Cell-Free DNA (cfDNA) Purification Kit contains sufficient reagents to isolate cell-free DNA from 100 ml or 250 ml human plasma or serum. Easily process input volumes from 100 µl to 10 ml. Both fresh and frozen plasma can be used, however, fresh plasma tends to have higher yields. Plasma from blood collected with Streck Cell-Free DNA BCT® Tubes must go through a Proteinase K treatment (not included) prior to purification to ensure optimal yields.

The Cell-Free DNA (cfDNA) Purification Kit is shipped and can be stored at room temperature. Please confirm receipt of all reagents upon arrival and store items at the appropriate temperatures as indicated below. All reagents are guaranteed stable for 6 months from date of receipt when stored properly. This kit includes the following components:

  • Lysis/Binding Buffer AM2; Store at RT

  • Wash Buffer AM8; Store at RT

  • Elution Buffer AM6; Store at RT

  • Magnetic Bead Solution; Store at RT.

Cell-Free DNA Purification Kit Highlights

  • Fast magnetic bead-based purification protocol that is compatible with automation

  • Suitable for a wide range of input volumes - 100 µl to 10 ml

  • Purify high-quality cfDNA from fresh or frozen samples

  • Concentrate your cell-free DNA with low elution volumes

  • Compatible with Streck Cell-Free DNA BCT® Tubes.