Cas9 ELISA Kit

Code: PRB-5079

The Cas9 protein (CRISPR associated protein 9) has gained attention as a genome engineering tool to cause site-directed double strand breaks in DNA. Resulting DNA breaks can inactivate genes or introduce heterologous genes through non-homologous end joining and homologous recombination, respectively, in many model organisms. Both gene deletion and gene replacement have been demonstrated using the CRISPR/Cas9 system in human cells.

Our Cas9 ELISA Kit is an immunoassay developed for the detection and quantitation of S. pyogenes Cas9 in lysates made from cells or tissues. Unknown samples are compared against a Cas9 standard curve. Each kit provides sufficient reagents for up to 96 assays including standards and unknown samples.

Kit Components:

Box 1 (shipped at room temperature)

1. Anti-Cas9 Antibody Coated Plate (Part No. 50791B): One 96-well strip plate (8 x 12).

2. Biotinylated Anti-Cas9 Antibody (1000X) (Part No. 50792C): One 10 µL vial.

3. Streptavidin-Enzyme Conjugate (Part No. 310803): One 20 µL vial.

4. Assay Diluent (Part No. 310804): One 50 mL bottle.

5. 10X Wash Buffer (Part No. 310806): One 100 mL bottle.

6. Substrate Solution (Part No. 310807): One 12 mL amber bottle.

7. Stop Solution (Part. No. 310808): One 12 mL bottle.

Box 2 (shipped on blue ice packs)

1. Cas9 Standard (Part No. 50793D): One 50 µL vial of 5 µg/mL recombinant S. pyogenes Cas9.

Recent Product Citations

1. Van Cleemput, J. et al. (2021). CRISPR/Cas9-Constructed Pseudorabies Virus Mutants Reveal the Importance of UL13 in Alphaherpesvirus Escape from Genome Silencing. J Virol. 95(6):e02286- 20. doi: 10.1128/JVI.02286-20.

2. Lee, M.H. et al. (2021). Cellular reprogramming with multigene activation by the delivery of CRISPR/dCas9 ribonucleoproteins via magnetic peptide-imprinted chitosan nanoparticles. Mater Today Bio. 9:100091. doi: 10.1016/j.mtbio.2020.100091.