Pure, Unsupported Nitrocellulose Membrane for Nucleic Acid and Protein Detection. High binding capacity for proteins and nucleic acids

Lower protein burnthrough than competitors in electrophoretic transfers. Prestained proteins were separated in a polyacrylamide gel and electrophoretically transferred to the indicated nitrocellulose membranes. A double layer of membrane was used, one directly against the gel, followed by the second layer. Signal intensity on the second layer is indicative of burnthrough, which can lead to loss of signal.