ATAC-Seq Kit

ATAC-Seq Kit

Code: 53150

ATAC-Seq is a rapid assay that allows analysis of epigenetic profiles across the genome by identification of regions that have open or accessible chromatin states. Because of the assay’s speed, simplicity, and applicability to a wide range of sample types, ATAC-Seq has become a commonly-used epigenetic assay, and it can serve as a gateway to further, more detailed epigenetic analyses.

In the ATAC-Seq assay, intact nuclei from cell or tissue samples are treated with a hyperactive Tn5 transposase mutant. This transposase is able to simultaneously tag the target DNA with sequencing adapters and fragment the DNA in a process termed tagmentation.

The ATAC-Seq Kit from Active Motif provides the reagents necessary to produce 16 unique sequencing-ready Illumina®-compatible ATAC-Seq libraries from 20 – 30 mg tissue or 50,000 – 100,000 cells per reaction. The optimized protocol guides you through the steps for sample preparation, tagmentation and library preparation, yielding next-gen sequencing-ready libraries that can be multiplexed in a single flow cell sequencing run.

The ATAC-Seq Kit is shipped at two temperatures, with one box on dry ice for components to be stored at -20°C, and a second box at room temperature for components to be stored at 4°C. Please store components according to the storage conditions in the manual after first use. All reagents are guaranteed stable for 6 months from date of receipt when stored properly.

Reagents included:

  • ATAC Lysis Buffer, store at RT
  • Assembled Transposomes, store at -20°C
  • 2X Tagmentation Buffer, store at -20°C
  • 1X PBS, store at -20°C or RT
  • 10X PBS, store at RT
  • 10% Tween 20, store at RT
  • 1% Digitonin, store at -20°C
  • DNA Purification Columns, store at RT
  • DNA Purification Binding Buffer, store at RT
  • DNA Purification Wash Buffer, store at RT
  • DNA Purification Elution Buffer, store at RT
  • 3 M Sodium Acetate, store at RT
  • 10 mM dNTPs, store at -20°C
  • 5X Q5 Buffer, store at -20°C
  • Q5 High-Fidelity DNA Polymerase, store at -20°C
  • i7 Indexed Primer 1, store at -20°C
  • i7 Indexed Primer 2, store at -20°C
  • i7 Indexed Primer 3, store at -20°C
  • i7 Indexed Primer 4, store at -20°C
  • i5 Indexed Primer 1, store at -20°C
  • i5 Indexed Primer 2, store at -20°C
  • i5 Indexed Primer 3, store at -20°C
  • i5 Indexed Primer 4, store at -20°C
  • SPRI Beads, store at 4°C.

ATAC-Seq Kit Highlights:

  • Reveal the genomic sequence of open chromatin regions

  • Yields next-gen sequencing-ready libraries in hours

  • Simple and rapid optimized protocol & reagents.